Abstract
Background Spleen-Kidney Supplementing Formula (SKSF), composed of 6 raw Chinese herbs and proposed based on the therapeutic principle of supplementing spleen-kidney and clearing the extra heat of stomach-lung, is effective in the treatment of type 2 diabetes mellitus (T2DM). AimThis study aims to investigate the mechanism of SKSF to alleviate renal fibrosis in diabetic rats. MethodsT2DM model was induced by high-fat diet and multiple injections of low-dose streptozotocin. After 8-week intervention, samples were collected for detection. ResultsSKSF decreased fasting blood glucose, glycosylated hemoglobin A1c, blood urea nitrogen, uric acid, urea, 24-hour urine protein, and KW/BW ratio, while it increased creatinine clearance rate of T2DM rats. Meanwhile, SKSF attenuated the renal fibrosis and improved the morphology and structure of renal tissue. Furthermore, SKSF significantly reduced the expression level of plasma miR-21 and TGF-β1 protein level and increased PTEN protein level. ConclusionSKSF could attenuate the renal damage and renal fibrosis induced in T2DM, which may be related to its regulation on the expressions of TGF-β1, PTEN, and miR-21.
Highlights
Type 2 diabetes mellitus (T2DM) is an increasing public health issue and its prevalence has been growing at a high rate [1, 2]
Primary antibodies of TGF-β1 and PTEN were from Beyotime Institute of Biotechnology (Shanghai, China)
At the end of the experiment, the concentrations of fasting blood glucose (FBG), hemoglobin A1c (HbA1c), blood urea nitrogen (BUN), uric acid (UA), urea, 24-hour urine protein excretion, and KW/BW ratio increased significantly, while clearance rate (CCR) decreased in type 2 diabetes mellitus (T2DM) rats
Summary
Type 2 diabetes mellitus (T2DM) is an increasing public health issue and its prevalence has been growing at a high rate [1, 2]. Treatment for T2DM patients with DN is challenging, because the decline in renal function impairs the clearance and metabolism of antidiabetic agents and insulin, requiring frequent reassessment of prescriptions. Spleen-Kidney Supplementing Formula (SKSF), composed of 6 raw Chinese herbs and proposed based on the therapeutic principle of supplementing spleen-kidney and clearing the extra heat of stomach-lung, is effective in the treatment of type 2 diabetes mellitus (T2DM). Aim. This study aims to investigate the mechanism of SKSF to alleviate renal fibrosis in diabetic rats. SKSF could attenuate the renal damage and renal fibrosis induced in T2DM, which may be related to its regulation on the expressions of TGF-β1, PTEN, and miR-21
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