Abstract
This study investigated the effect of spirulina ethanolic extract (SEE) on the quality of ram semen during low-temperature liquid storage and the relationship between sperm features. Ejaculates were collected from five Djallonké rams, pooled, extended with Tris-egg yolk (TEY) enriched with 0 (control), 20 (SEE20), 40 (SEE40), and 80 µg/mL (SEE80) of SEE to reach the concentration of 200×106 spz/mL, and stored at 4 °C for 72 h. Extended semen samples were assessed for total motility, progressive motility, sperm motion characteristics, viability, membrane integrity, and morphology at 6, 24, 48 and 72 h of storage. Moreover, malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase, (SOD) and catalase (CAT) levels were measured at 72 h of storage. The enrichment of TEY with SEE at 40 and 80 µg/mL, improved sperm total motility at 48 and 72 h of storage (P
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