Spirosoma swuense sp. nov., isolated from wet soil.

Abstract

Strain JBM2-3T, a pale-yellow-coloured, aerobic, catalase-negative, oxidase-positive and Gram-stain-negative bacterium, was isolated from wet soil. The isolate grew aerobically at 25-30 °C (optimum 25 °C), pH 6.0-8.0 (optimum pH 7.0) and in the presence of 0-0.5 % (w/v) NaCl (optimum 0 % NaCl). Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain JBM2-3T belonged to the genus Spirosoma, with a sequence similarity of 96.2 % with Spirosoma panaciterrae Gsoil 1519T. The strain showed the typical chemotaxonomic characteristics of the genus Spirosoma, with the presence of menaquinone 7 as the respiratory quinone; the major fatty acids were summed feature 3 (composed of C16: 1ω6c/ω7c), C16: 1ω5c and iso-C15: 0. The DNA G+C content of strain JBM2-3T was 47.4 mol%. The polar lipid profile contained major amounts of phosphatidylethanolamine and aminophospholipids. On the basis of its phenotypic and genotypic properties, and phylogenetic distinctiveness, strain JBM2-3T should be classified as a representative of a novel species in the genus Spirosoma, for which the name Spirosoma swuense sp. nov. is proposed. The type strain is JBM2-3T (=KCTC 52176T=JCM 31298T).