Abstract
Previous studies showed that rabbit muscle phosphofructokinase-1 (PFK-1) activity losses due to dilution, due to inhibition by ascorbate, and due to some lithium salts were prevented by rabbit muscle aldolase. Chicken PFK-1 and fish PFK-1 interacted with ascorbate and were inhibited, consistent with a previously proposed function that ascorbate facilitates glycogen in resting muscle by inhibiting glycolysis. This report shows that a plant enzyme, spinach aldolase, has the same ability to prevent rabbit muscle PFK-1 activity loses as rabbit muscle aldolase and in some instances it was a better protector from activity losses than rabbit aldolase. Spinach aldolase also protected chicken and fish PFK-1s from inhibitions by ascorbate and from activity losses due to dilution. Prevention of losses PFK-1 activities from animal species by a plant protein, spinach aldolase, suggests an evolutionary conservative relationship between PFK-1s and aldolases.
Highlights
Influenced by several metabolic intermediates, phosphofructokinase-1 (PFK-1) is the putative controlling enzyme of glycolysis
Because brain depends on glycolysis as an energy source and because lithium salts are used therapeutically in manic-depressive syndrome [4,5,6,7,8,9], another purpose was to study the ability of Li2CO3 to inhibit rabbit muscle PFK-1
Neither cm PFK-1 nor fm PFK-1 were so well protected from activity losses due to dilution by rm aldolase as was rm PFK-1 [2] where it was shown that dilutions below 200 nM rm PFK-1 resulted in tetramer dissociations to less active dimers or monomers
Summary
Influenced by several metabolic intermediates, phosphofructokinase-1 (PFK-1) is the putative controlling enzyme of glycolysis. Compounds of potential therapeutic value were examined to determine their influence on PFK-1 activity. A report on the anti-metastatic effect of. The effects of AA on muscle PFK-1 from three animal species, mammal, bird, and fish, were studied. A purpose of this study was to further demonstrate or not the role of AA as a facilitator of glycogen synthesis in resting muscle [2,3]. Because brain depends on glycolysis as an energy source and because lithium salts are used therapeutically in manic-depressive syndrome [4,5,6,7,8,9], another purpose was to study the ability of Li2CO3 to inhibit rabbit muscle (rm) PFK-1. Because spinach (sp) aldolase and rm aldolases share several characteristics, comparison of rm aldolases and sp aldolase interactions with mammalian PFK-1s was undertaken; the abilities of rm aldolase and sp aldolase were compared for their abilities to prevent inhibitions of rm PFK-1
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