Spin state selective Hadamard encoding during transfer periods using multiple selective CW-HCP

Abstract

Hadamard spectroscopy today represents an alternative to conventional Fourier transform spectroscopy. The selective inversion of several narrow frequency bands is typically achieved by taylored inversion pulses in place of t 1-evolution periods. However, band-selective inversion can also be achieved during coherence transfer steps, thereby shortening the period during which the magnetization is in the transverse plane. Using CW heteronuclear cross polarization (CW-HCP) as an example for highly selective coherence transfer, the implementation of Hadamard encoding within a transfer step is presented. Transfer characteristics, the preparation of multiple frequency selective CW-HCP and the possibility of acquiring spin state selective spectra are discussed in detail. The theoretical results are verified on two examples involving a cyclic pentapeptide and ubiquitin.