Abstract
It is demonstrated that a new pulse sequence element, Spin-State-SelectiveCoherence Transfer (S3CT), via an intermediate state ofheteronuclear IS zero- or double-quantum coherence can transfer the twosingle-quantum coherences on one of the spins exclusively to any one of thetwo single-quantum coherences on the other spin. This fact is used for editinginto two subspectra that are most suitable for extraction of homo- orheteronuclear J coupling constants when S3CT is combined withhomonuclear coherence transfer during a mixing period. Experimentalconfirmation is obtained using a 15N-labeled 58-residue protein,the C-terminal Kunitz domain from human type VI collagen. The J coupling con-stants determined include 3JHN-Hα and3JN-Hβ related to the φ andχ1 angles, respectively.
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