Abstract
The effects of weak magnetic fields on the biological production of reactive oxygen species (ROS) from intracellular superoxide (O2 •−) and extracellular hydrogen peroxide (H2O2) were investigated in vitro with rat pulmonary arterial smooth muscle cells (rPASMC). A decrease in O2 •− and an increase in H2O2 concentrations were observed in the presence of a 7 MHz radio frequency (RF) at 10 μTRMS and static 45 μT magnetic fields. We propose that O2 •− and H2O2 production in some metabolic processes occur through singlet-triplet modulation of semiquinone flavin (FADH•) enzymes and O2 •− spin-correlated radical pairs. Spin-radical pair products are modulated by the 7 MHz RF magnetic fields that presumably decouple flavin hyperfine interactions during spin coherence. RF flavin hyperfine decoupling results in an increase of H2O2 singlet state products, which creates cellular oxidative stress and acts as a secondary messenger that affects cellular proliferation. This study demonstrates the interplay between O2 •− and H2O2 production when influenced by RF magnetic fields and underscores the subtle effects of low-frequency magnetic fields on oxidative metabolism, ROS signaling, and cellular growth.
Highlights
One of the greatest challenges in the field of chemical and physical biology is to bridge the knowledge gap between the atomic level and the cellular level [1]
This study demonstrates the interplay between O2N2 and H2O2 production influenced by radio frequency (RF) magnetic fields and underscores the subtle effects of low-frequency magnetic fields on oxidative metabolism, reactive oxygen species (ROS) signaling, and cellular growth
We observed a slight correlation with Paraquat reduction in O2N2 and H2O2 trends; we expect a significant increase in H2O2 with RF and diphenyleneiodonium chloride (DPI)
Summary
One of the greatest challenges in the field of chemical and physical biology is to bridge the knowledge gap between the atomic level and the cellular level [1]. Focused at the biological quantum/classical interface, an emerging field called quantum biology has promised to offer new and compelling insights into fundamental underlying cellular processes from the perspective of quantum phenomena [2,3]. Following this paradigm, we present a novel methodology for indirectly investigating possible quantum effects in biological systems by applied static and alternating magnetic fields that induce changes in magnetically sensitive free radical pairs in biochemical reactions. The effect of weak magnetic fields on cellular metabolic processes is not well understood and little is known about how magnetic fields influence reaction rates in oxidative metabolism [8,9,10]. This work aims to elucidate biological responses that are sensitive to radio frequency (RF) magnetic fields involving the production of reactive oxygen species (ROS), of which are born presumably from spin-correlated free radical pairs
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