Sphingosine-1-Phosphate Receptor Type 4 (S1P4) Is Differentially Regulated in Peritoneal B1 B Cells upon TLR4 Stimulation and Facilitates the Egress of Peritoneal B1a B Cells and Subsequent Accumulation of Splenic IRA B Cells under Inflammatory Conditions.

Janik Riese,Annabel Kleinwort,Felix Lührs,Alina Gromann,Tobias Schulze

doi:10.3390/ijms22073465

Abstract

Background: Gram-negative infections of the peritoneal cavity result in profound modifications of peritoneal B cell populations and induce the migration of peritoneal B cells to distant secondary lymphoid organs. However, mechanisms controlling the egress of peritoneal B cells from the peritoneal cavity and their subsequent trafficking remain incompletely understood. Sphingosine-1-phosphate (S1P)-mediated signaling controls migratory processes in numerous immune cells. The present work investigates the role of S1P-mediated signaling in peritoneal B cell trafficking under inflammatory conditions. Methods: Differential S1P receptor expression after peritoneal B cell activation was assessed semi‑quantitatively using RT-PCR in vitro. The functional implications of differential S1P1 and S1P4 expression were assessed by transwell migration in vitro, by adoptive peritoneal B cell transfer in a model of sterile lipopolysaccharide (LPS)‑induced peritonitis and in the polymicrobial colon ascendens stent peritonitis (CASP) model. Results: The two sphingosine-1-phosphate receptors (S1PRs) expressed in peritoneal B cell subsets S1P1 and S1P4 are differentially regulated upon stimulation with the TLR4 agonist LPS, but not upon PMA/ionomycin or B cell receptor (BCR) crosslinking. S1P4 deficiency affects both the trafficking of activated peritoneal B cells to secondary lymphoid organs and the positioning of these cells within the functional compartments of the targeted organ. S1P4 deficiency in LPS-activated peritoneal B cells results in significantly reduced numbers of splenic innate response activator B cells. Conclusions: The S1P-S1PR system is implicated in the trafficking of LPS-activated peritoneal B cells. Given the protective role of peritoneal B1a B cells in peritoneal sepsis, further experiments to investigate the impact of S1P4-mediated signaling on the severity and mortality of peritoneal sepsis are warranted.

Highlights

Expression related to the function of the stimulating signal, sphingosine-1phosphate receptors (S1PRs) expression was assessed at the mRNA level after peritoneal B cell activation with three different activation stimuli: (a) nonspecific stimulation with phorbol myristate acetate (PMA) and ionomycin, (b) crosslinking of the B cell receptor using agonistic anti-mouse IgM, and (c) specific toll-like receptor 4 (TLR4) stimulation with LPS [8]
S1P1 and S1P4 were the two S1P receptor subtypes expressed in peritoneal B cells, while S1P2, S1P3, and S1P5 were not detectable in all three subtypes of peritoneal B cells (B1a, B1b, and B2; resting or activated) at a level greater than the background signal
While thelymph molecular governing the passage from of lymphocytes the blood compartment into the nodeevents and the exit of lymphocytes the lymph from the blood compartment into the lymph node and the exit of lymphocytes from the node have been studied in detail, there remains considerable scientific debate on the lymph node have been studied in detail, there remains considerable scientific debate on the mechanisms involved in the exit of lymphocytes from body cavities and the subsequent localization of these cells in secondary lymphoid organs (SLOs) [31]

Summary

Introduction

Among the peritoneal lymphocyte populations, B1 B cells serve a pivotal role in the pathogenesis of abdominal sepsis and constitute approximately 35–70% of CD19+ B cells in the peritoneal cavity [6]. Gram-negative infections of the peritoneal cavity result in profound modifications of peritoneal B cell populations and induce the migration of peritoneal B cells to distant secondary lymphoid organs. Methods: Differential S1P receptor expression after peritoneal B cell activation was assessed semi-quantitatively using RT-PCR in vitro. Results: The two sphingosine-1phosphate receptors (S1PRs) expressed in peritoneal B cell subsets S1P1 and S1P4 are differentially regulated upon stimulation with the TLR4 agonist LPS, but not upon PMA/ionomycin or B cell receptor (BCR) crosslinking.

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