Abstract
Our aim was to verify the alleviation effect of sphingosine-1-phosphate (S1P) in a miniature pig model. Thirty male miniature pigs were randomly separated into 10 groups in our experiment. We administered S1P through the parotid duct in a retrograde fashion 2hr before irradiation (IR). The salivary flow rate and blood flow rate were tested 20weeks after IR. The apoptotic level was checked at 12, 24hr and 7days post-IR. Twenty weeks after IR, the salivary flow rate of the IR-side parotid gland in IR+S1P group can be maintained at about 40% of the non-IR side, while only 20% was maintained in the IR group. The blood flow rate and microvascular density were significantly higher in the IR+S1P group than in the IR group. The apoptotic level and cleaved caspase-3 expression were downregulated in IR+S1P group, and the ratio of Bcl-2/Bax was increased. The blood flow rate and CD31 level were significantly restored at 12, 24hr and 7days post-IR. Sphingosine-1-phosphate may partially alleviate IR-induced parotid dysfunction by decreasing apoptosis of microvascular endothelial cells and maintaining the blood flow rate.
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