Sphingomonas liriopis sp. nov., Sphingomonas donggukensis sp. nov., and Sphingomonas tagetis sp. nov., isolated from Liriope platyphylla fruit, soil, and Tagetes patula roots

Abstract

Three bacterial strains, designated RP10T, RMG20T, and MG17T, were isolated from Liriope platyphylla fruit (strain RP10T), soil (RMG20T), and Tagetes patula roots (MG17T) collected in Goyang, Republic of Korea. The 16S rRNA gene sequences revealed that strains RP10T, RMG20T, and MG17T were closely related to Sphingomonas melonis DSM 14444T (highest similarity of the strain RP10T), Sphingomonas asaccharolytica DSM 10564T (strain RMG20T), and Sphingomonas suaedae JCM 33850T (strain MG17T) with 98.0-99.0% highest sequence similarity. The 16S rRNA gene sequences similarity between strains RP10T, RMG20T, and MG17T was 96.6-97.4%. Strains RP10T, RMG20T, MG17T, and the closely related type strains have digital DNA-DNA hybridization and average nucleotide identity values of 19.4-65.3% and 74.0-95.7%, respectively. Based on phylogenetic, biochemical, chemotaxonomic, and phenotypic data, strains RP10T, RMG20T, and MG17T are considered to represent novel species of the genus Sphingomonas, for which the name Sphingomonas liriopis sp. nov. (type strain RP10T = KACC 22357T = TBRC 15161T), Sphingomonas donggukensis sp. nov. (type strain RMG20T = KACC 22358T = TBRC 15162T), and Sphingomonas tagetis sp. nov. (type strain MG17T = KACC 22355T = TBRC 15160T), are proposed.