Sphingomicrobium nitratireducens sp. nov., isolated from a tidal flat in Guangxi.

Abstract

An aerobic, yellow-pigmented and Gram-stain-negative strain, designated as O-35T, was isolated from a tidal flat sediment collected in Dangjiang Town, the southern China. Colonies of strain O-35T were circular with 0.5-1.0mm in diameter, convex and smooth. Cells of strain O-35T were coccoid-shaped, non-spore forming, non-motile and the strain could reduce nitrate. Growth of strain O-35T was observed at 15-40°C (optimum 30°C), at pH 6.0-9.5 (optimum 7.5-8.0) and in 0.5-5.0% NaCl (optimum 2%, w/v). Strain O-35T showed 16S rRNA gene sequence identities of 97.3-97.5% with Sphingomicrobium lutaoense CC-TBT-3T and Sphingomicrobium aestuariivivum AH-M8T, higher than the rest of Sphingomicrobium type strains. Phylogenetic trees based on the 16S rRNA gene and the core-genome sequences demonstrated that strain O-35T was affiliated within the genus Sphingomicrobium. Overall genome relatedness index calculations revealed that strain O-35T had < 75.8% of average nucleotide identity and < 19.2% of digital DNA-DNA hybridization values with Sphingomicrobium type strains. The sole isoprenoid quinone was ubiquinone-10. The major fatty acids (> 10%) were summed feature 8, summed feature 3, C16:0 and C18:1 2-OH. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, sphingoglycolipid, two unidentified glycolipids, one unidentified lipid and one unidentified phospholipid. On the basis of the phenotypic, chemotaxonomic and genomic properties, strain O-35T represents a novel species in the genus Sphingomicrobium, for which the name Sphingomicrobium nitratireducens sp. nov. is proposed. The type strain is O-35T (= KCTC 92308T = MCCC 1K07589T).