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Abstract
B. melaninogenicus provides a unique system for the study of the biosynthesis of an important group of lipids, the phosphosphingolipids. Sphingolipid biosynthesis can be repressed and induced by depletion and restoration of vitamin K. At least one enzyme involved in sphingolipid biosynthesis from the microorganism can be solubilized and so purified by conventional methods. Pathways involved in biosynthesis may differ from hitherto postulated pathways, for example, the incorporation of NH4+ into ethanolamine residue of ceramide phosphorylethanolamine. Moreover, the derivation of mutants defective in steps in sphingolipid biosynthesis would be of great value in these studies.
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