Sphingobium faniae sp. nov., a pyrethroid-degrading bacterium isolated from activated sludge treating wastewater from pyrethroid manufacture

Abstract

A bacterial strain capable of degrading pyrethroid, designated JZ-2(T), was isolated from activated sludge treating pyrethroid-contaminated wastewater. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JZ-2(T) belongs to the genus Sphingobium. It showed the highest levels of 16S rRNA gene sequence similarity to Sphingobium cloacae JCM 10874(T) (98.3 %) and Sphingobium ummariense CCM 7431(T) (97.1 %), and 94.8-96.9 % similarity to the type strains of other members of the genus Sphingobium. Strain JZ-2(T) contained C(18 : 1)omega7c as the predominant fatty acid, C(14 : 0) 2-OH as the major 2-hydroxy fatty acid, ubiquinone Q-10 as the main respiratory quinone, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine and two sphingoglycolipids as the predominant polar lipids and spermidine as the major polyamine. DNA-DNA hybridization results showed that strain JZ-2(T) had low genomic relatedness with S. cloacae JCM 10874(T) (34 %) and S. ummariense CCM 7431(T) (23 %). Based on the phenotypic, genotypic and phylogenetic data presented, strain JZ-2(T) is considered to represent a novel species of the genus Sphingobium, for which the name Sphingobium faniae sp. nov. is proposed. The type strain is JZ-2(T) (=CGMCC 1.7749(T) =DSM 21829(T)).