Sphingobacterium yanglingense sp. nov., isolated from the nodule surface of soybean.

Abstract

A Gram-staining-negative, non-motile, catalase- and oxidase-positive strain, designated CCNWSP36-1(T), was isolated from the nodule surface of soybean [Glycine max (L.) Merrill] cultivar Zhonghuang 13. The 16S rRNA gene sequence analysis clearly showed that the isolate represented a member of the genus Sphingobacterium. On the basis of pairwise comparisons of 16S rRNA gene sequences, strain CCNWSP36-1(T) showed 96.8% similarity to Sphingobacterium nematocida CCTCC AB 2010390(T) and less than 95.2% similarity to other members of the genus Sphingobacterium. Growth of strain CCNWSP36-1(T) occurred at 10-40 °C and at pH 5.0-9.0. The NaCl range (w/v) for growth was 0-4%. The predominant isoprenoid quinone was MK-7. The polar lipids were phosphatidylethanolamine and several unidentified polar lipids. Sphingolipid was present. The major fatty acids were iso-C(15:0) and summed feature 3 (comprising C(16:1)ω6c and/or C(16:1)ω7c). The G+C content of the genomic DNA was 41.1 mol%. As the physiological and biochemical characteristics of strain CCNWSP36-1(T) and the type strains of its closest phylogenetic neighbours showed clear differences, a novel species, Sphingobacterium yanglingense, is proposed. The type strain is CCNWSP36-1(T) ( =ACCC 19328(T) =JCM 30166(T)).