Abstract

A special arrangement of electrondense cylindrical bodies has been observed in the cytoplasm of trophozoites of Entamoeba histolytica. In a previous report we communicated its presence in trophozoites of E. histolytica cultivated in monoxenic medium. We called this structure “centriole-like body”, which seems to be inadequate in base of the present work. In the progress of this study and examining large amounts of trophozoites cultivated in mixed, monoxenic and axenic culture media, we have observed the spherical structure of cylindrical bodies in the cytoplasm of the trophozoites, regardless the nutrient elements present in the medium. In order to see the detailed structure of the components of this organelle, very thin sections, lightly stained with lead citrate of osmium-fixed material were examined. Some sections were observed unstained and other trophozoites were fixed in glutaraldehyde and incubated in Gomori's medium for acid phosphatase. Electrondense cylindrical units scattered throughout the cytoplasm were seen in all trophozoites examined.

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