Spermine induced endogenous signal ameliorates osmotic stress in Lens culinaris.

Abstract

The present study evaluates the alleviation of polyethylene glycol (PEG) mediated osmotic stress by exogenous spermine (SP) application on seeds of Lens culinaris. Seeds were soaked overnight with SP (0.05M) and PEG (10%) separately. Third set was prepared by combinedtreatment with SP (0.05M) and PEG (10%). Further, various physiological parameters, defense enzymes, phenol, flavonoid, nitric oxide (NO), proline content, chromosomal abnormality studies etc. was conducted after 5days of incubation. PEG application significantly hampered the seed germination, root length, shoot length, relative water content and vigour index. Application of PEG induced electrolytic leakage in rootcells. PEG application also significantly reduced the activities of different defense parameters viz. peroxidase, polyphenol oxidase, phenylalanine ammonia lyase and β-1,3 glucanase, total phenol and flavonoid accumulation over control. Reactive oxygen species, lipid peroxidation, cell death was found significantly high in PEG treated sets. However, NO level has been significantly decreased by PEG treatment. Cytological studies showed reduced mitotic index and highest abnormalities under osmotic stress. Contrary to that, application of SP either alone or in combination with PEG showed improved responses of aforesaid defense parameters compared to control and solely PEG treated sets. Interestingly, cytological abnormalities were also reduced in SP treated roots. Overall, these results suggests that SP application on seeds reduced the PEG induced damage and intricately improved the defense mechanisms which might help to fight against oxidative stress.