Spermatogonium compartment in experimental administration of semicarbazide

Abstract

Lathyrism is a pathologic condition with deficient collagen synthesis, which can be induced in the lab animal by the administration of semicarbazide.The aim of this study is to evaluate the seminiferous tubules morphologic changes, measuring the area occupied by the spermatogonia, in the experimental administration of semicarbazide.Thirty Wistar male rats, 8 weeks‐old were randomly distributed on two groups: the control group was kept without manipulation; the animals of test group were submitted to the administration of semicarbazide incorporated in the diet in the dose of 6g/kg, during 4 weeks. All the animals were sacrificed on the 30th day, by an overdose of anaesthetics and the complete necropsy was performed.The fragments collected in the necropsy were studied under light microscopy, stained by Haematoxylin & Eosin. The testicle's slides were submitted to morphometric analysis to evaluate the area occupied by spermatogonium compartment.The statistical analysis by frequencies distribution allows observing different distributions of the different cases. Comparing both groups, we observe more cases with medium area than on the test group. The graphic of the test group presents a peak of cases with greater areas than the control ones.These results allow us to conclude that the spermatogenesis is changed on the administration of semicarbazide.