Spermatogonia of rainbow trout: II. in vitro study of the influence of pituitary hormones, growth factors and steroids on mitotic activity.

Abstract

At the present time, in spite of recent advances, knowledge about the factors regulating germ cell proliferation in the teleost testis is limited. This study was designed to investigate, in vitro, the ability of various hormones, growth factors, and steroids to influence the proliferation of trout spermatogonia (Go) present in mixed cultures of somatic and germ cells prepared from testes, either prespermatogenetic or spermatogenetic. The tested molecules were usually present for the duration of culture (4.5 days) and 3H-thymidine (3H-Tdr) for the last day in culture. In our cell culture conditions, homologous gonadotropin I (tGTH-I) and growth hormone (tGH) moderately stimulated 3H-Tdr incorporation by Go, with ED50 equal to 5.5 +/- 3.0 and 1.8 +/- 0.4 ng/ml respectively. Insulin growth factor I (rhIGF-I) and fibroblast growth factor (rhFGF-2) stimulated 3H-Tdr incorporation by Go from spermatogenetic testes only, with ED50 equal to 16.2 +/- 9.3 and 2.4 +/- 0.3 ng/ml respectively. The effects of the most efficient concentrations of rhIGF-I combined with those of either tGTH-I or tGH were additive. Seventy to one hundred microM suramin stimulated 3H-Tdr incorporation by Go from testes at all maturation stages and this effect was additive with that of tGTH-I. We assume that this effect of suramin could result from the inhibition of an unidentified antimitogenic factor. No effect was observed with homologous prolactin, human epidermal growth factor, activin A and B, transforming growth factor-beta1, testosterone, 11-ketotestosterone, 17beta-estradiol, pregnenolone, 11beta-hydroxyprogesterone, and 22-hydroxycholesterol. In conclusion, our in vitro results suggest that GTH-I, GH, IGF-I, and FGF-2, are potent in situ modulators of the proliferative activity of trout Go at the time of induction, speeding up, then slowing down spermatogenesis, through direct or indirect additive and/or antagonistic influences.