Spermatogenesis in the Roborovski hamster (Phodopus roborovskii) and the Chinese hamster (Cricetulus griseus).

Abstract

Spermatogenesis is an elaborately organized and tightly regulated differentiation process. The spermatogenesis duration is stable within a certain species but highly variable between species of the same family. In this study, the spermatogenesis duration of the Roborovski hamster was measured for the first time, and the spermatogenesis duration of the Chinese hamster was re-assessed. Stage classification and cycle length measurement were carried out by labeling the dividing cells with bromodeoxyuridine and an antibody-based chromogen as well as with the periodic acid-Schiff/hematoxylin stain. Analysis was conducted using reference calculation and linear regression. Morphological measurements completed our set of methods. The mean duration of one seminiferous epithelium cycle was 8.58±0.34days (mean±SEM; Phodopus roborovskii) and 16.59±0.47days (Cricetulus griseus) based on the reference calculation. Slightly higher results were obtained using linear regression analysis: 9.72±0.41days for P.roborovskii and 17.64±0.61days for C.griseus. Additionally, a newly developed exemplary flowchart was proposed for the Roborovski hamster to facilitate spermatogenesis stage classification also in other species. The Chinese hamster presented an unexpectedly high paired epididymides weight of 1.701±0.046g (mean±SEM) although having a body weight of only 40.5±0.7g. However, no significant correlation between the relative epididymis weight and spermatogenesis duration in mammals (Spearman rank correlation: r=-0.119, p=0.607, n=21) or rodents could be found (r=0.045, p=0.903, n=11). Our data emphasize the stability of the spermatogenesis duration within species and its remarkable variability between species. Further research is needed to identify the principal mechanisms and selection drivers that are responsible for such stability within species and the variability between species.