Abstract
The activities of phosphoglycerate kinase B and lactate dehydrogenase C4 were correlated with the state of spermatogenesis in testes of sex-reversed (Sxr) mice. Both isoenzymes were present in Sxr/+,XO testes, which contained late spermatids. LDH-C4 was detected in testes from 5 of 9 Sxr/+,XX mice, but PGK-B was present in testes of only 1 of the 5 mice. Pachytene spermatocytes were the predominant cell types in those Sxr/+,XX testes that contained spermatogenic cells. These observations confirm that PGK-B is restricted to spermatogenic cells and is not present in somatic testicular cells. Furthermore, they suggest that PGK-B synthesis may be initiated later than pachytene, when synthesis of the LDH-C polypeptide is known to commence.
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