Abstract
The present study aims to evaluate the vitrification of the sperm of Prochilodus brevis. Six pools were made and diluted in media composed of 5% glucose (Experiment 1) or Powder Coconut Water (ACP-104; Experiment 2) associated with dimethylsulphoxide (DMSO) or ethylene glycol (EG), or its association (DMSO + EG), in concentrations of 10%, 20% or 30%. Diluted samples were vitrified, stored in liquid nitrogen, devitrified after 15 days and evaluated for kinetics, morphology and plasmatic membrane (PMI) and sperm DNA (SDI) integrity. The experiments were conducted and evaluated independently of each other. No differences (p > 0.05) were found for the kinetics and morphology parameters in both experiments. The other parameters recorded interaction between cryoprotectants and their concentrations. The best PMI results (p < 0.05) were for glucose with 10% EG (19.75 ± 2.40) and ACP-104 with 30% EG (46.92 ± 4.59). The best SDI rates (p < 0.05) were observed when glucose (45.43 ± 5.07) and ACP-104 (71.83 ± 3.62) were associated with 30% EG. It is possible to conclude that vitrification has great potential for P. brevis sperm conservation; thus, we suggest using the association between ACP-104 and 30% EG.
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