Abstract
Abstract This study aimed to evaluate the vitrification method for Litopenaeus vannamei sperm cryopreservation by using soy lecithin as extracellular cryoprotectant. For the toxicity assay three intracellular cryoprotectants were tested: dimethyl sulfoxide (DMSO), ethylene glycol (EG) and methanol (MeOH), at the final concentrations of 5, 10, or 30% ( v/v ) using sterile calcium-free saline solution (Ca-F) as the extender medium. After extrusion, the sperm masses were immersed in 0.5 mL of cryoprotectant solutions for 10, 30 and 120 min. Next, apparent sperm viability (ASV) was assessed by eosin–nigrosin stain technique. Methanol was selected due to its minimal reduction ( L. vannamei sperm mass. Apparently, this was the first report of a penaeid sperm mass cryopreservation by the vitrification method.
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