Sperm quality of Bali bull following sexing and freezing using different cryoprotectants

Abstract

The objective of this experiment was to assess the impact of sperm sexing duration on sperm quality and to compare the effectiveness of glycerol (GC) and ethylene glycol (EG) as cryoprotectants to preserve the quality of sexed sperm during the freezing and storage in liquid nitrogen. The treatments consisted of three different durations of sexing: 40 min.(D-40), 50 min (D-50), 60 min. (D-60). The variable measured was the motility and viability of sperm following sexing and post-thawing motility of Bali bull sexed sperm cryopreserved in GC and EG. The results showed that sperm motility after sexing was significantly higher in the treatment of 40 and 50 min. compared to 60 min. of sexing duration. While the viability of sexed sperm in all treatments was not significantly different. Post-thawing motility of frozen sexed sperm cryopreserved with GS was higher than sexed sperm cryopreserved with EG. Finally, it was concluded that sexing times of 40 and 50 min. were the best time to get high sperm motility and viability of Bali bull compared to sexing times of 60 min. In addition, GS could maintain the motility of sexed sperm better than EG.