Sperm motility in baseline spermogram is predictive of total motile sperm after preparation for insemination

Abstract

ObjectiveEvery institution has diferent cut-off values to determine which is the required amount of total motile sperms after preparation for intrauterine insemination. In our center we consider 3 million total motile sperm as the minimum necesary to consider the sample as appropiate. The objective of this study is to determine whether sperm motility in baseline spermogram is predictive of total motile sperm count after sperm preparation for insemination.DesignRetrospective, descriptive analysis.Materials and methodsPatients presenting to the University Center of Reproductive Medicine at “Hospital Universitario” in Monterrey, Mexico for sperm preparation between July 2004 and June 2006. Spermogram parameters were recorded according to WHO guidelines. Swim-up and density gradients preparations were included. ROC curve was used to determine the amount of total motile sperm in the baseline evaluation were necesary to obtain more than 3 millions motile sperm after preparation.ResultsSamples prepared with densitiy gradient technique showed a braking point at 4.03 million sperms/ml for a sensitivity of 90.7% (95% CI 77.8%-97.3%) and a specificity of 70.59% (95% CI 44.1%-89.6%). The PPV was 95% and NPV 54.9%.Figure 1View Large Image Figure ViewerDownload Hi-res image Download (PPT)ConclusionsTotal motile sperm count in baseline spermogram can be used as a good predictor for and adecuate total motile sperm count after sperm preparation for insemination with swim up or density gradient technique. ObjectiveEvery institution has diferent cut-off values to determine which is the required amount of total motile sperms after preparation for intrauterine insemination. In our center we consider 3 million total motile sperm as the minimum necesary to consider the sample as appropiate. The objective of this study is to determine whether sperm motility in baseline spermogram is predictive of total motile sperm count after sperm preparation for insemination. Every institution has diferent cut-off values to determine which is the required amount of total motile sperms after preparation for intrauterine insemination. In our center we consider 3 million total motile sperm as the minimum necesary to consider the sample as appropiate. The objective of this study is to determine whether sperm motility in baseline spermogram is predictive of total motile sperm count after sperm preparation for insemination. DesignRetrospective, descriptive analysis. Retrospective, descriptive analysis. Materials and methodsPatients presenting to the University Center of Reproductive Medicine at “Hospital Universitario” in Monterrey, Mexico for sperm preparation between July 2004 and June 2006. Spermogram parameters were recorded according to WHO guidelines. Swim-up and density gradients preparations were included. ROC curve was used to determine the amount of total motile sperm in the baseline evaluation were necesary to obtain more than 3 millions motile sperm after preparation. Patients presenting to the University Center of Reproductive Medicine at “Hospital Universitario” in Monterrey, Mexico for sperm preparation between July 2004 and June 2006. Spermogram parameters were recorded according to WHO guidelines. Swim-up and density gradients preparations were included. ROC curve was used to determine the amount of total motile sperm in the baseline evaluation were necesary to obtain more than 3 millions motile sperm after preparation. ResultsSamples prepared with densitiy gradient technique showed a braking point at 4.03 million sperms/ml for a sensitivity of 90.7% (95% CI 77.8%-97.3%) and a specificity of 70.59% (95% CI 44.1%-89.6%). The PPV was 95% and NPV 54.9%. Samples prepared with densitiy gradient technique showed a braking point at 4.03 million sperms/ml for a sensitivity of 90.7% (95% CI 77.8%-97.3%) and a specificity of 70.59% (95% CI 44.1%-89.6%). The PPV was 95% and NPV 54.9%. ConclusionsTotal motile sperm count in baseline spermogram can be used as a good predictor for and adecuate total motile sperm count after sperm preparation for insemination with swim up or density gradient technique. Total motile sperm count in baseline spermogram can be used as a good predictor for and adecuate total motile sperm count after sperm preparation for insemination with swim up or density gradient technique.