Abstract
Infertility is a common condition affecting one in six couples of childbearing age. In approximately 40% of these cases, a male factor is involved. Sperm DNA integrity is essential for accurate transmission of genetic information. Materials and Methods: In this study 152 patients, 64 patients it is infertility group and 88 patients are fertile males. The ejaculate samples were taken in accordance with the patient to whom the reason for the analysis of the ejaculate sample was previously explained. All patients have been in the Dukagjini Region in the Republic of Kosovo. The samples were collected from 2016/18. Sperm Chromatin Dispersion (SCD) test, analysis in the ejaculate was performed at the Biolab Zafi, Laboratory in Peja, in the Republic of Kosovo. Statistical analysis: Data are reported as mean ± SD. The comparisons between groups were tested by student's t-test, ANOVA. A p-value less than 0.05% was considered statistically significant. Results: From our research studies, we have achieved significant (p <.00001) scores among the working group and control group across all sperm parameters, and DNA fragmentation. Conclusion: In summary, we have demonstrated that there was a negative correlation between DNA fragmentation, sperm motility, and morphology in infertile males. We conclude that sperm DNA fragmentation appears to be a useful technique to predict outcome in couples undergoing IVF/ICSI. To evaluate whether DFI 23.94 ± 4.68% can be used to determine male infertility in our country by Sperm Chromatin Dispersion (SCD), it is necessary to carry out further large-scale research by other authors.
Highlights
Infertility is a common condition affecting one in six couples of childbearing age
We investigated the prevalence of sperm DNA damage in a group of infertile men with both normal and abnormal semen parameters in order to elucidate whether Sperm Chromatin Dispersion (SCD) analysis can add to the information obtained by routine semen analysis in explaining the causes of infertility
Sperm Chromatin Dispersion (SCD) test allows for an improved evaluation of sperm quality, complementing the information offered by the conventional sperm analysis
Summary
Infertility is a common condition affecting one in six couples of childbearing age. In approximately 40% of these cases, a male factor is involved. DNA damage in spermatozoa, and subsequent effects of such damage on male reproductive ability, is one of these factors which has been studied in the past few years Because half of the offspring’s DNA is originating from paternal unit, it is of utmost importance to consider the detrimental effects of sperm chromatin and DNA damage on reproduction (Marchettini et al, 2007). Semen analysis is a routine test to evaluate sperm quality. Semen analysis is subjective and sperm parameters vary based on patient conditions. They do not always reveal the quality and health of sperm (Agarwal et al, 2003). Reactive oxygen species (ROS) and abnormalities in chromatin packaging could be major major sources of sperm DNA damage
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