Sperm characteristics as additional evidence of close relationship between Lebiasina and Piabucina (Characiformes: Lebiasinidae: Lebiasininae)

Júlio César De O Santana,Daniela Calcagnotto,André L Netto-Ferreira,Irani Quagio-Grassiotto

doi:10.1590/s1679-62252013000300010

Júlio César De O Santana, Daniela Calcagnotto + Show 2 more

Open Access

https://doi.org/10.1590/s1679-62252013000300010

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Abstract

Spermiogenesis and spermatozoa of representatives of the genera Lebiasina and Piabucinaare described. Spermiogenesis is quite similar among all analyzed species of these genera and is identified as Type II. In this type of spermiogenesis, the flagellum of earliest spermatids lies lateral to the nucleus. A slight movement of the nucleus towards the centriolar complex is observed. In late spermatids, the nucleus slightly elongates towards the flagellum. The formation of two striated rootlets apparently occurs in early/intermediate spermatids. The spermatozoa of species of Lebiasinaand Piabucina share (1) a drop-shaped slightly elongated nucleus that is laterally positioned relative to the flagellum; (2) a superolateral centriolar complex; (3) two striated rootlets; (4) a basolateral midpiece; (5) oblong mitochondria and (6) a few spherical vesicles. The spermatic characteristics of Lebiasinaand Piabucinaindicate that spermiogenesis is a homologous process among all species of both genera examined to date and can be considered, along with the spermatozoa morphology, additional evidence indicating a close relationship between Lebiasina and Piabucina.

Highlights

Members of the family Lebiasinidae are known from Costa Rica and Panama in Central America and all countries of South America except Chile
Descriptions of spermatic characteristics are based on analyses at the ultrastructural level of gametic cells from testis of adult males of Lebiasina aff. festae (MUSM 6530), L. bimaculata (MUSM 15931), L. erythrinoides (MCNG 41376), L. melanoguttata (MZUSP 99610), L. aff. uruyensis1 (MZUSP 109118), L. aff. uruyensis2 (MHNLS 14468), Piabucina boruca (ANSP 140657), P. elongata (ANSP 130567) and P. panamensis (ANSP 104137)
Details of spermiogenesis are consistent across the species of the Lebiasininae included in this study; we present a general description of the process for all species

Summary

Introduction

Members of the family Lebiasinidae are known from Costa Rica and Panama in Central America and all countries of South America except Chile. Most species of the family are found in quiet, clear, or black water streams from sea level to about 250 meters elevation. Some species of the Lebiasininae, on the other hand, occur to over 1000 meters elevation The Lebiasinidae is divided into two subfamilies, the Lebiasininae and Pyrrhulininae. The Lebiasininae is currently composed of 26 valid species, traditionally allocated to two genera, Lebiasina and Piabucina. Lebiasina has been distinguished from Piabucina based on the lack of an adipose fin and by the cellular composition of the swim bladder wall in Lebiasina versus the presence of an adipose fin and a noncellular

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