Speed of sound and acoustic attenuation of compounds affected during optoacoustic monitoring of thermal therapies measured in the temperature range from 5°C to 60°C

Abstract

Optoacoustic (photoacoustic) imaging is being adopted for monitoring tissue temperature during hypothermic and hyperthermic cancer treatments. The technique’s accuracy benefits from the knowledge of speed of sound (SoS) and acoustic coefficient of attenuation (AcA) as they change with temperature in biological tissues, blood, and acoustic lens of an ultrasound probe. In these studies we measured SoS and AcA of different <i>ex vivo </i>tissues and blood components (plasma and erythrocyte concentrates) in the temperature range from 5&deg;C to 60&deg;C. We used the technique based on measurements of time-delay and spectral amplitude of pressure pulses generated by wideband planar acoustic waves propagating through the interrogated medium. Water was used as a reference medium with known acoustic properties. In order to validate our experimental technique, we measured the temperature dependence of SoS and AcA for aqueous NaCl solution of known concentration and obtained the results in agreement with published data. Similar to NaCl solution and pure water, SoS in blood and plasma was monotonously increasing with temperature. However, SoS of erythrocyte concentrates displayed abnormalities at temperatures above 45°C, suggesting potential effects from hemoglobin denaturation and/or hemolysis of erythrocytes. On the contrary to aqueous solutions, the SoS in polyvinyl-chloride (plastisol) – a material frequently used for mimicking optical and acoustic properties of tissues – decreased with temperature. We also measured SoS and AcA in silicon material of an acoustic lens and did not observe temperature-related changes of SoS.