Abstract
Purpose: To investigate the spectrum-effect relationships between high performance liquid chromatography with photodiode array detection (HPLC-DAD) fingerprint and analgesic activity of Anisodus tanguticus (Maxim.) Pascher (Solanaceae) (AT) roots.Methods: Analgesic activity of AT roots was evaluated by acetic acid-induced writhing test in mice. Fingerprint of AT roots was established by HPLC-DAD. After oral administration of AT roots extract, intra-gastric contents of caffeoylputrescine, anisodine, fabiatrin, scopolin, scopolamine, anisodamine and atropine in mice were determined by HPLC-DAD. Spectrum-effect relationships between HPLCDAD fingerprint and analgesic activity were investigated using bivariate correlation analysis.Results: Following treatment with different batches of AT roots extract, acetic acid-induced writhing responses in mice were inhibited significantly (p < 0.05 or 0.01), with inhibitions of 26.62 - 55.13 %, relative to the control group. Sixteen common peaks were obtained by fingerprint analysis. Peaks 1, 2, 6, 7, 8, 9 and 12 were identified as caffeoylputrescine, anisodine, fabiatrin, scopolin, scopolamine, anisodamine and atropine, respectively. Bivariate correlation analysis between analgesic activity of AT roots and 16 common peaks areas indicated the contributions of 16 common peaks to analgesic activity of AT roots. Surprisingly, bivariate correlation analysis between analgesic activity of AT roots and intragastric contents of above-named 7 constituents revealed that the contributions of the 7 constituents to analgesic activity of AT roots were different from those based on their peak areas.Conclusion: This study provides scientific justification for the investigation of the active constituents of AT root with a view to its standardization.Keywords: Anisodus tanguticus root, Analgesic activity, HPLC-DAD fingerprint, Bivariate correlation analysis
Highlights
The main chemical constituents of Anisodus tanguticus (Maxim.) Pascher (Solanaceae) (AT)roots are alkaloids, flavonoids, coumarins and phenolcarboxylic acid [1]
These results showed that the method was suitable and feasible for determination of the contents of the test constituents
The spectrum-effect relationships between analgesic activity and HPLC-DAD fingerprint of AT roots were investigated in this study for the first time
Summary
The main chemical constituents of Anisodus tanguticus (Maxim.) Pascher (Solanaceae) (AT)roots are alkaloids, flavonoids, coumarins and phenolcarboxylic acid [1]. Existing reports about AT roots are primarily focused on contents of the main alkaloids anisodamine, anisodine, scopolamine and atropine [2,3,4]. It is recorded in Chinese Tibetan medicine and China flora that AT roots exhibit sedative, analgesic and antitussive activities, but there have been no studies to confirm these pharmacologic effects [1,5]. AT roots have been used as raw material in the extraction of tropane alkaloids for a long time [5]. To avoid wastage of AT roots during extraction of tropane alkaloids, a feasible solution is to promote their application as medicinal herbs for treating diseases. Quality standards of medicinal herbs in Chinese Pharmacopoeia show that quantitative determinations of active constituents are very important in assessing the quality of medicinal herbs [7]
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