Abstract
Yangyin Tongnao (YYTN) prescription is used as a traditional Chinese herbal formula, and it has antioxidant activity that mainly contributes in the treatment of cardiovascular and cerebrovascular diseases. However, the compounds related to its antioxidant activity are still unknown. In the present study, the fingerprints of YYTN extracts under different extraction conditions were obtained by high performance liquid chromatography (HPLC) to identify the common peaks to all the samples processed. A 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity assay and ferric reducing antioxidant power (FRAP) assay were carried out to evaluate the antioxidant activity of the extracts. Spectrum-effect relationship between HPLC fingerprints and antioxidant activity of YYTN was assessed by Pearson product-moment correlation coefficient (PPMCC) and multiple linear regression analysis (MLRA). The results showed that peaks 5, 6, 13, 15, and 24 of the fingerprints were closely connected to antioxidant activity. Five peaks were identified: vanillic acid (P5), puerarin (P7), ferulic acid (P13), daidzein (P21), and formononetin (P23). Our study successfully established the spectrum-effect relationship between HPLC fingerprints and antioxidant activity of YYTN, which provided a general method for establishing quality standards with a combination of chromatography and antioxidant activity.
Highlights
Yangyin Tongnao prescription is a traditional Chinese herbal formula composed of six kinds of Chinese herbal medicines, Radix Rehmanniae, Radix Astragali, Puerariae Lobatae Radix, Ligustici Chuanxiong Rhizoma, Dendrobii Caulis, and Hirudo
DPPH Assay. e IC50 values of DPPH radical scavenging activity test are listed in Table 7. e results revealed that nine extracts of Yangying Tongnao (YYTN) solution exhibited the IC50 values that appeared within the range of 3.023–4.396 mg/mL
Pearson product-moment correlation coefficient (PPMCC) analysis is performed with peak areas of twentyfour peaks as independent variables and IC50 values of DPPH radical scavenging percentage and ferric reducing antioxidant power (FRAP) values as dependent variables
Summary
Yangyin Tongnao prescription is a traditional Chinese herbal formula composed of six kinds of Chinese herbal medicines, Radix Rehmanniae, Radix Astragali, Puerariae Lobatae Radix, Ligustici Chuanxiong Rhizoma, Dendrobii Caulis, and Hirudo. Four kinds of these Chinese herbal medicines except Dendrobii Caulis and Hirudo have been studied as a simplified formula in a lot of research [1, 2]. As a traditional Chinese herbal formula, YYTN can improve the neurological deficits after focal cerebral ischemic infarction in rats [3]. It was found that YYTN has protective effect on oxidative stress-induced cerebral ischemia injury in vivo through animal experiments [7]. There does not Journal of Analytical Methods in Chemistry
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