Spectrum of Aerobic Bacterial Pathogen isolated from Pus samples and their Antibiotic Sensitivity Pattern in a Tertiary Care Hospital

Abstract

Background:Wound infection continues to be a significant problem in clinical practice where empirical treatment of infection is required while waiting for culture report to prevent emergence of resistance. Objectives:To study the spectrum of aerobic bacterial pathogen isolated from pus samples and to determine the antimicrobial sensitivity pattern of the bacteria isolated. Method: The samples of pusreceived in Clinical Microbiology Laboratory were processed during the study period to isolate and identify bacterial pathogens as per standard bacteriologicaltechniques. Antibiotic susceptibility testing was done by Kirby Bauer disc diffusion method as per CLSI guidelines 2017. Result:Out of the total of 1474pus samples processed, 21.98% samples were culture positive. Majority of culture positive samples were from IPD patients.Predominance of gram negative bacteria (77.4%) was seen as compared to gram positive bacteria (22.60%) both in IPD and OPD samples. High level of resistance to various antimicrobials was seen among the clinical isolates. Conclusion: Emergence of antibiotic resistant strains in pus sample is a matter of concern. Therefore, knowledge of the spectrum of microorganisms causing pus discharge and its susceptibility pattern is required and this data may contribute to an effective management of cases of wound infection