Spectrum-Effect Relationships Between Chemical Fingerprints and Antibacterial Effects of Lonicerae Japonicae Flos and Lonicerae Flos Base on UPLC and Microcalorimetry.

Zhilong Shi,Zhenjie Liu,Jiabo Wang,Haibin Su,Chunsheng Liu,Mingquan Wu,Yanling Zhao,Xiaohe Xiao,Xiao Ma,Yimei Zang

doi:10.3389/fphar.2016.00012

Abstract

The traditional Chinese medicines Lonicerae Japonicae Flos (LJF, Jinyinhua in Chinese) and Lonicerae Flos (LF, Shanyinhua in Chinese) refer to the flower buds of five plants belonging to the Caprifoliaceae family. Until 2000, all of these were officially listed as a single item, LJF (Jinyinhua), in the Chinese Pharmacopoeia. However, there have recently been many academic controversies concerning the separation and combination of LJF and LF in administrative regulation. Till now there has been little work completed evaluating the relationships between biological activity and chemical properties among these drugs. Microcalorimetry and UPLC were used along with principal component analysis (PCA), hierarchical cluster analysis (HCA), and canonical correlation analysis (CCA) to investigate the relationships between the chemical ingredients and the antibacterial effects of LJF and LF. Using multivariate statistical analysis, LJF and LF could be initially separated according to their chemical fingerprints, and the antibacterial effects of the two herbal drugs were divided into two clusters. This result supports the disaggregation of LJF and LF by the Pharmacopoeia Committee. However, the sample of Lonicera fulvotomentosa Hsu et S. C. Cheng turned out to be an intermediate species, with similar antibacterial efficacy as LJF. The results of CCA indicated that chlorogenic acid and 3,4-Dicaffeoylquinic acid were the major components generating antibacterial effects. Furthermore, 3,4-Dicaffeoylquinic acid could be used as a new marker ingredient for quality control of LJF and LF.

Highlights

Lonicerae Japonicae Flos (LJF, Lonicera japonica Thunb., Jinyinhua in Chinese) is one of the most commonly used traditional Chinese medicines
The results of hierarchical cluster analysis (HCA) showed that the extracts of LJF and LF from different origins had similar chemical fingerprints, yet HCA could initially separate the LJF and LF samples at the chemical level
Nowadays the strategy for quality control of herbal drugs depends mostly on measuring the content of constituents, which is correlated with the safety and efficacy of herbal medicine (Capasso et al, 2000)

Summary

Introduction

Lonicerae Japonicae Flos (LJF, Lonicera japonica Thunb., Jinyinhua in Chinese) is one of the most commonly used traditional Chinese medicines. LJF is officially listed in the 2015 version of the Chinese pharmacopeia, where it is listed simultaneously with Lonicerae Flos (LF, Shanyinhua in Chinese). They have the same descriptions of flavor, meridian tropism, functions, dosage, and indications in the pharmacopeia (Commission, 2015). Before the 2000 version of the Chinese pharmacopoeia, both LJF and LF were listed under the same category of LJF (Jinyinhua in Chinese). Is listed in LJF (Jinyinhua in Chinese) in the 2015 version of the Chinese pharmacopoeia, while the other plant sources are listed in LF (Shanyinhua in Chinese). It is urgent to do further research to determine the pharmaceutical activity of the two herbal drugs

Methods

Results

Conclusion