Spectrum-Effect Relationship between UPLC Fingerprints and Antilung Cancer Effect of Si Jun Zi Tang.

Xiaowei Zhou,Qiong Gu,Mingyu Zhang,Wei Chen,Guohui Li,Bin Dong,Chunyu Li,Ying Li,Haiyang Liu,Yafei Shi,Junjie Hao,Yuanyuan Zhang

doi:10.1155/2019/7282681

Abstract

Nowadays, an increasing number of patients are seriously affected by lung cancer. Si Jun Zi Tang (SJZ), a four-herb Chinese medicine formula first described approximately one thousand years ago, is often prescribed for cancer patients as a complementary therapy. But the research on the effective materials for treating cancer using SJZ was rarely reported. To solve this problem, we evaluate the inhibitory effect of 10 samples of SJZ from different origins on PC9 cells. Ultraperformance liquid chromatography (UPLC) and hierarchical cluster analysis (HCA) along with canonical correlation analysis (CCA) and bioactivity validation were used to investigate the underlying correlation between the chemical ingredients and the antiproliferative effect of SJZ on PC9 cells. The evaluation indicated that 10 batches of SJZ could inhibit proliferation of PC9 cells and there was a notable difference in pharmacological activity between the different SJZ samples. The results of CCA and multivariate statistical analysis indicated that ginsenoside Ro and ginsenoside Rg1 might be active constituents of the antiproliferative effect as determined by spectrum-effect relationships. The results showed that bioassay and spectrum-effect relationships are suitable to associate sample quality with the active ingredient associated with clinical efficacy. And our finding would provide foundation and further understanding of the quality evaluation of traditional Chinese medicine decoction.

Highlights

Lung cancer is the leading cause of cancer-related mortality worldwide. 1.8 million people are diagnosed with lung cancer, and 1.6 million people die from this disease every year [1, 2]
The deeper color indicates the peak area of the Si Jun Zi Tang (SJZ) samples of higher intensity. e results suggested that the content of chemical constituents contained in samples in cluster 1 was higher than that in cluster 2. e differences in the chemical ingredients could be related to the different geographic locations. e results showed that the SJZ samples from different origins had similar chemical fingerprint, and hierarchical cluster analysis (HCA) could initially separate the samples at the chemical level
Traditional Chinese medicines (TCMs) decoction is an oral liquid preparation prepared by decocting several Chinese herbal medicines according to the theory of “Jun-Chen-Zuo-Shi” and appropriate dosage ratio

Summary

Introduction

Lung cancer is the leading cause of cancer-related mortality worldwide. 1.8 million people are diagnosed with lung cancer, and 1.6 million people die from this disease every year [1, 2]. Lung cancer is the leading cause of cancer-related mortality worldwide. 1.8 million people are diagnosed with lung cancer, and 1.6 million people die from this disease every year [1, 2]. Malignant proliferation of tumor cells is one of the leading causes of death. Traditional Chinese medicines (TCMs) have been used to treat a variety of tumor with the obvious advantages of fewer side effects [3, 4]. As a representative formula for tonic TCM, SJZ has the effect of replenishing qi and strengthening spleen and is usually used to regulate gastrointestinal function, anti-inflammatory, and enhances immunity [5, 6]. The chemical composition of SJZ against lung cancer remains unclear, and it is difficult to control the quality linked to the clinical effects

Methods

Results

Conclusion