Abstract
This review emphasises the need to use spectroscopy in order to understand the behaviour of water, and summarises the background of the subject. The various forms of spectroscopy that are especially informative are described, with particular reference to near-infrared (NIR) spectrophotometry. The key results are outlined, first those obtained with small molecules and ions, and second those involving proteins, DNA and cell membranes. Finally, some interpretations are offered which include the novel but possibly controversial concept of free OH and free lone-pair groups.
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