Abstract
We have developed a full-field optical coherence microscopy system providing intensity-based tomographic images and spectroscopic information with ultrahigh spatial resolution. Local measurements of the backscattered light spectrum center of mass are achieved through short-time Fourier analysis of a stack of en face interferometric images acquired with a Linnik-type microscope. Using a halogen lamp as an illumination source enables us to achieve spectroscopic imaging over a wavelength range from 600 to 900 nm with a spatial resolution of approximately 1 microm. Absorption measurements of a colored gel are reported as a validation of the technique. Enhancement of tissue imaging contrast is demonstrated by imaging a Xenopus Laevis (African frog) tadpole ex vivo.
Highlights
Full-Field Optical Coherence Microscopy (FF-OCM), referred to as Full-Field Optical Coherence Tomography (FF-OCT), is an efficient technique for ultrahigh-resolution (~1 μm) three-dimensional imaging of biological media [1,2,3,4]
We have developed a full-field optical coherence microscopy system providing intensity-based tomographic images and spectroscopic information with ultrahigh spatial resolution
Spectroscopic OCT is an extension of conventional scanning OCT that is used for performing cross-sectional tomographic imaging and spectroscopic imaging simultaneously [10,11,12]
Summary
Full-Field Optical Coherence Microscopy (FF-OCM), referred to as Full-Field Optical Coherence Tomography (FF-OCT), is an efficient technique for ultrahigh-resolution (~1 μm) three-dimensional imaging of biological media [1,2,3,4]. FF-OCM was proposed recently as an alternative method to conventional Optical Coherence Tomography (OCT) [5,6,7]. It uses a CCD camera as an array detector in combination with a spatially and temporally incoherent illumination source for parallel acquisition of en face-oriented (transverse) tomographic images. Information on the spectral content of backscattered light is obtained by detection and processing of the OCT interferometric signal This method allows the spectrum of backscattered light to be measured over the entire available optical bandwidth, which can extend from 650 nm to 1000 nm using ultrabroad-band Ti:saphire laser technology [11]
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