Abstract
Background We developed an automated, multiwell plate (MWP) based screening system for suspension cell cultures which is now routinely used in cell culture process development. It is characterized by a fully automated workflow with integrated analytical instrumentation. It uses shaken 6-24 well plates as bioreactors which can be run in batch and fed-batch mode with a capacity of up to 768 reactors in parallel [1,2]. A wide ranging analytical portfolio is available to monitor cell culture processes and also a cooperation with internal high throughput (HT) analytic groups to characterize product quality. The current work focuses on expanding the analytical portfolio to develop control mechanisms for automated cell culture processes. Besides setting up a robust method for pH measurement we evaluated different spectroscopic techniques like Raman or infrared as fast and powerful analytical tools.
Highlights
We developed an automated, multiwell plate (MWP) based screening system for suspension cell cultures which is routinely used in cell culture process development
Besides setting up a robust method for pH measurement we evaluated different spectroscopic techniques like Raman or infrared as fast and powerful analytical tools
As mentioned before for the Infrared spectroscopy, the low concentrations of the target components are in combination with the complex cell culture media a problem for the Raman spectroscopy, too
Summary
Background We developed an automated, multiwell plate (MWP) based screening system for suspension cell cultures which is routinely used in cell culture process development. A wide ranging analytical portfolio is available to monitor cell culture processes and a cooperation with internal high throughput (HT) analytic groups to characterize product quality. The current work focuses on expanding the analytical portfolio to develop control mechanisms for automated cell culture processes. Besides setting up a robust method for pH measurement we evaluated different spectroscopic techniques like Raman or infrared as fast and powerful analytical tools.
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