Abstract
Excitation spectroscopy with Opto-Thermal Transient Emission Radiometry (OTTER) was used to measure waterkeratin interaction energies in excised stratum corneum (SC) under controlled ambient relative humidity (RH) conditions. Such interactions lead to small but measurable wavelength shifts in the peaks of the absorption spectra of the interacting molecules. In the present study, an OPO laser with tuneable output radiation in the wavelength range 2.4-3.4µm was used to measure spectral peak shifts of the prominent absorption band of water near 3µm wavelength. A thermal emission wavelength around 13.1µm was selected by means of an interference filter, to reject emissions not emanating from water. Peak shifts within three types of SC samples, namely untreated, delipidized and delipidized & rinsed, with RH values in the range 3%-100% were measured. This new method of measuring interaction energies is unique in its potential for non-invasive in-vivo and in-situ measurements of water binding within the stratum corneum.
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