Spectroscopic Study Of Thin Multilaver Films Of The Complexes Of Nucleic Acids With Cationic Amphiphiles And Polycations: Their Possible Use As Sensor Elements

Abstract

SUMMARY Multilayer films of DNA complexes with octadecylamine (ODA) and dioctadecyldimethylammonium (DODA) were obtained using LangmuirBlodgett technique (LB-films) and the films of DNA complexes with polyallylamine, polyethylenimine, po1y-Llysine and poly-L-arginine using a successive alternating adsorption method (SA films) IR spectral studies showed that DNA is unfolded in the multilayer LB-films of its complex with ODA but preserves its double helical structure in the LB-films with DODA and in the SA-films with polycations. The atomic groups and the types of interactions that determine the formation of these films were identified. The hydration of LB- and SA-films was studied and the binding sites of water molecules were determined. The possibility of using such films as sensor elements on nucleic acid-tropic molecules was discussed.