Abstract
Non-structural carbohydrates (NSC) are products of photosynthesis, and leaf NSC concentration may be a prognostic indicator of climate-change tolerance in woody plants. However, measurement of leaf NSC is prohibitively labor intensive, especially in tropical forests, where foliage is difficult to access and where NSC concentrations vary enormously by species and across environments. Imaging spectroscopy may allow quantitative mapping of leaf NSC, but this possibility remains unproven. We tested the accuracy of NSC remote sensing at leaf, canopy and stand levels using visible-to-shortwave infrared (VSWIR) spectroscopy with partial least squares regression (PLSR) techniques. Leaf-level analyses demonstrated the high precision (R2 = 0.69–0.73) and accuracy (%RMSE = 13%–14%) of NSC estimates in 6136 live samples taken from 4222 forest canopy species worldwide. The leaf spectral data were combined with a radiative transfer model to simulate the role of canopy structural variability, which led to a reduction in the precision and accuracy of leaf NSC estimation (R2 = 0.56; %RMSE = 16%). Application of the approach to 79 one-hectare plots in Amazonia using the Carnegie Airborne Observatory VSWIR spectrometer indicated the good precision and accuracy of leaf NSC estimates at the forest stand level (R2 = 0.49; %RMSE = 9.1%). Spectral analyses indicated strong contributions of the shortwave-IR (1300–2500 nm) region to leaf NSC determination at all scales. We conclude that leaf NSC can be remotely sensed, opening doors to monitoring forest canopy physiological responses to environmental stress and climate change.
Highlights
Non-structural carbohydrates (NSC) are the mobile portion of a plant’s carbon stock, comprised primarily of sugars, starch and pectin [1,2]
The variation expressed in these spectra meets or exceeds the variation reported for leaf optical properties in other biomes [29,30,31,32,33,34] and matches the reported variability achieved in models of leaf optical properties [19,35]
Using a three-tier strategy, we determined that leaf NSC concentrations can be estimated using high-fidelity spectroscopy at leaf, canopy and whole-stand scales with demonstrably high precision and accuracy compared to laboratory-based chemical assays
Summary
Non-structural carbohydrates (NSC) are the mobile portion of a plant’s carbon stock, comprised primarily of sugars, starch and pectin [1,2]. Known as non-structural carbon or soluble carbon, plant NSC are produced and stored in leaves and can be transported to and stored in stems and roots. Plant NSC stocks reflect a balance between carbon fixation via photosynthesis and demand for longer-lasting compounds, such as cellulose and lignin. NSC measurements provide biochemically-based insight into physiological performance (carbon “source”) relative to whole plant growth (carbon “sink”) [3,4]. Recent work reveals that NSC are an important determinant of tree survival during drought [6]. O’Brien et al [6]
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