Abstract
Hyperthermophilic pretreatment composting (HPC) is superior to traditional composting (CK) with shortened maturity period and enhanced humification degree. However, the chemical and structural evolution of humic substances (HS) at the molecular level is not known. In this study, the impact of hyperthermophilic pretreatment (90 °C, 4 h) on the content and chemical composition of HS during composting were investigated. The HS content of the final compost was 87.8 g/kg and 76.7 g/kg in HPC and CK, respectively. Significantly higher humic acid/fulvic acid ratio (1.27 in HPC v.s. 0.77 in CK) was observed in HPC. 13C NMR spectroscopic data showed a higher aromatics percentage and earlier enrichment of aromatic structures in HS extracted from HPC than CK. Intensified humification of HPC was related to the increased levels of HS precursors and degradation of lignocellulose. Redundancy analysis demonstrated that aromatic C, phenolic C and O-alkyl C can be used for evaluation of the humification degree.
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