Spectroscopic characteristics of water-extractable organic matter from different green manures1

Abstract

ABSTRACT Water-extractable organic matter (WEOM) plays an important role in many chemical processes and in soil organic matter accumulation. Large amounts of WEOM can be released by green manure when being incorporated into the soil. However, the characteristics of WEOM extracted from different green manures (GMs) are unclear. In this study, WEOM samples were extracted from Chinese milk vetch (Astragalus sinicus L), radish (Raphanus sativus L), ryegrass (Lolium perenne L), hairy vetch (Vicia villosa L), February orchid (Orychophragmus violaceus) and rye (Secale cereale L) and their characteristics were studied by spectroscopic analysis. WEOM generated from legume GMs contained more water-extractable organic carbon (WEOC) than other GMs and Chinese milk vetch was the highest. UV-visible spectroscopy analysis revealed that all the samples were rich in C=C and C=O functional groups. Carboxylic acid, alcohol, phenol, fatty acids, aliphatic aromatic and amide compounds were found by FTIR spectroscopy in these WEOM samples. WEOM derived from Chinese milk vetch contained more carboxylic acid and inorganic sulphates only appeared in WEOM extracted from the radish. The aromaticity index, humification index, and FTIR absorption ratio 1650/2925 and 1650/2850 showed that WEOM extracted from Chinese milk vetch and hairy vetch had a relatively lower aromaticity and humification degree than other GMs. The fluorescence regional integration (FRI) analysis showed that Chinese milk vetch and hairy vetch had a higher portion of protein-like substance fractions than other GMs. We may deduce that WEOM generated from Chinese milk vetch and hairy vetch has higher chemical activity than other GMs.