Spectroscopic and Electrochemical Studies of the Interaction Between Fuchsin Basic and DNA

Abstract

Visible spectroscopic and electrochemical methods were used to study the interactions between DNA and fuchsin basic (FB). FB has an irreversible electro-oxidation peak in 5 mmol/L Tris-HCl buffer solution at pH = 7. 4 on a glassy carbon electrode (GCE). After adding certain concentration of dsDNA, the oxidation peak current of FB decreases, but the peak potential hardly changes. The visible absorption spectroscopic study shows that the binding mode of FB to dsDNA is intercalative binding and electrostatic binding when the ratio of the concentration of dsDNA to FB is smaller than 0.2, and a new substance, which produces a new absorption peak, is obtained via a covalent binding between dsDNA and FB apart from intercalative binding and electrostatic binding when the ratio of the concentration of dsDNA to FB is larger than 0. 2. The visible absorption spectra varies no longer when the ratio of the concentration of dsDNA to FB is larger than 1. 5. A mean binding ratio of dsDNA to FB was determined to be 1. 4: 1, suggesting that two complexes FB-dsDNA and FB-2dsDNA be formed. The interaction between FB and ssDNA was only electrostatic binding. The more powerful interaction of FB with dsDNA than with ssDNA may be applied for the recognition of dsDNA and ssDNA, and in DNA biosensor as hybridization indicator.