SPECTROSCOPIC AND CHROMATOGRAPHIC CHARACTERISATION OF MUSHROOM EXTRACT (Pleurotus tuberregium) IN COMPARISON WITH SOME ANTIGLAUCOMA MEDICATIONS (0.5% TIMOLOL AND 0.005% LATANOPROST)

Abstract

Pleurotus tuberregium, an edible fungus, occurs in both tropical and subtropical regions of the world Scientific evidences exist for the use of P. tuberregium in the treatment of high blood pressure, diabetic hypertriglyceredemia, fungal and bacterial infections, tumours and raised intraocular pressure. However, its comparative chromatographic and spectroscopic analysis with anti-glaucoma medications has not been extensively explored. This study separated the bioactive constituents of the mushroom extract and compared it with 0.5% Timolol and 0.005% Latanoprost. Fractions of the extracts were obtained through Column chromatography utilizing silica gel. Retardation factors and migration speeds of the fractions were then obtained using Thin Layer Chromatography. UV-VIS spectrophotometry was then utilized to obtain a more refined result. The experiment produced comparative retardation factors and retardation factors of the extracts with those of the antiglaucoma medication. Spectroscopic studies on the extract revealed that it has an absorption spectrum within the ultra violet wavelength range with a λmax of 320nm. All spots for this study were produced with a reproducibility factor better than 1.5% RSD.