Spectroscopic analyses on interaction of melamine, cyanuric acid and uric acid with DNA

Abstract

In this work, the interaction of DNA with melamine (MEL), cyanuric acid (CYA) and uric acid (UA) were studied, respectively, by means of UV–vis, fluorescence, circular dichroism (CD) spectroscopy, viscosity and gel electrophoresis methods. The fluorescence quenching was used to study the interaction models of MEL, CYA and UA with DNA, respectively, and the bimolecular quenching constant (Kq), apparent quenching constant (Ksv), effective binding constant (KA) and corresponding dissociation constant (KD) and binding site number (n) were calculated by adopting Stern–Volmer, Lineweaver–Burk and Double logarithm equations. The results show that MEL, CYA and UA are all able to markedly bind to DNA, and the binding strength order is DNA–UA>DNA–CYA>DNA–MEL. It is wished that these researches would facilitate the understanding of the formation of kidney stones and gout in the body after ingesting excess MEL.