Abstract

1. 1. The absorptivity at 540 nm of hemiglobincyanide ( ε HiCN 540) from dog blood was determined on the basis of iron and found to be within the range formerly obtained for human hemoglobin. 2. 2. Consequently, ε HiCN 540 = 11.0, the established value for human hemoglobin, may be used for dog hemoglobin. 3. 3. On this basis the absorption spectra of oxyhemoglobin, de-oxygenated hemoglobin, carboxy-hemoglobin, hemiglobin (methemoglobin) and hemiglobincyanide were determined for dog hemoglobin. 4. 4. No significant differences were found between dog and human hemoglobin, except that dog hemiglobin binds less OH − as reflected in a difference between the absorption spectra of dog and human hemiglobin at the same pH.

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