Abstract

The absorption spectrum of hemoglobin, both in solution and in suspension of erythrocytes, is investigated by Shibata's Opal Glass transmission method.It is proved that the Soret band of erythrocyte suspension is flattened as compared with that of hemolysate although the position of the absorption maximum is identical with that of hemolysate and Beer's law is valid for the suspension.This flattening is due to the discrete distribution of hemoglobin molecules in the suspension (statistical effect).The degree of the flattening depends on the shape, size, and optical density of individual erythrocytes, and the relation observed among these properties can be explained, in first approximation, by Duysens'theory for suspensions of spherical particles.

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