Spectrophotometric Multi-Component Quantitation of Rosiglitazone, Glibenclamide and Metformin HCl in Pharmaceutical Dosages Form by using Cramer’s Matrix

Abstract

Objective: This article illustrates the development of a simple, precise and accurate spectrophotometric analysis method for the determination of Rosiglitazone, Glibenclamide and Metformin hydrochloride in their pharmaceutical preparations by using Cramer's matrix. Method: The absorbance of the sample solution was measured at 315.6nm and 229.2nm for the estimation of rosiglitazone and glibenclamide, respectively and at 237.0nm for estimation of metformin hydrochloride by Shimadzu double beam UV-visible spectrophotometer. Results: The result of the analysis for method was tested and validated for various parameters according to ICH guidelines and found satisfactory. The method was successfully applied for the determination of these drugs in their tablet dosage forms. Conclusion: Simultaneous estimation of active ingredients in multicomponent pharmaceutical products normally requires the use of chromatographic separation techniques, like thin layer chromatography, high performance thin layer chromatography, high performance liquid chromatography or gas chromatography etc., followed by their quantitation. But this article illustrates direct spectroscopic estimation of multicomponent formulation by using Cramer's matrix.