SPECTROPHOTOMETRIC METHODS FOR THE DETERMINATION OF FAMOTIDINE IN DRUG FORMULATIONS.

Abstract

This paper describes two sensitive and simple spectrophotometric methods for determination of famotidine in pharmaceutical preparations. The first method is based on the hydrolisis of famotidine with sodium hydroxide assisted with microwave radiation, whereby the sulfide ion is produced. The sulfide was allowed to react with N,N – diethyl – p – phenylendiamine oxalate and Fe(III), and the blue color produced was measured at 671 nm. Beer’s law was fulfilled in the concentration range 0.6 – 52.8 µg/mL, with a detection limit of 0.2 µg/mL. The second method is based upon the formation of a ternary complex between lead (II), eosin and famotidine in the presence of methyl cellulose as a surfactant. The ternary complex showed an absorption maximum at 541 nm. The method obeys Beer’s law over concentration range of 7.4-100.0 µg/mL. The methods are simple, sensitive and accurate. The proposed methods was applied on the famotidine determination in tablets and intravenous solution, shows recoveries in the range of 97.9-104.2 %. The results obtained by proposed methods were statistically validated.