Spectrophotometric method for the determination of chlordiazepoxide, diazepam and alprazolam drugs by using oxidation coupling reaction

Abstract

Sensitive, accurate, precise and efficient spectrophotometric"method for the determination of chlordiazepoxide, Diazepam and Alprazolam drug (CH, DIA, ALP) in pure as well as in dosage form was described."The suggested method was based on oxidation coupling reaction of suggested drug (CH, DIA, ALP) drug using acidic buffer "then"coupling with"3-methyl-2-benzothiazolinone hydrazone hydrochloride"(MBTH) in the presence of ferric chloride. Spectrophotometric measurement was established by recording the absorbance of the blue colored product at (663,609,619) nm. Using the optimized reaction conditions, beer’ s law was obeyed in the range of"(2.5-25) µg/mL "(5-30) µg/mL, and "(5-30) µg/mL with good correlation coefficient of (0.9997, 0.9979, 0.9965) and the LOD and LOQ ( CH,DIA,ALP) values were( 0.1750, 5.834 , 0.676 and 2.253 ,0.881and 2.938)μg/mL for chlordiazepoxide, Diazepam and Alprazolam drug respectively. The accuracy and precision of the proposed method represented by recovery and relative standard deviation were satisfactory; about 99.33% and)") 0.502and 0.195, 0.46) μg/mL for chlordiazepoxide, Diazepam and Alprazolam drug respectively. The "proposed"method"was"applied"for"determination"of (CH, DIA, ALP) in"its"pharmaceutical" "forms"and"the"results"compared successfully"with"the"obtained"by"standard"method (British"pharmacopeia"method).