Abstract
A continuous spectrophotometric assay modified for fungal pectinesterases is reported. This method is based on that developed by Hagerman and Austin for plant pectinesterases. The amount of carboxylic groups released during the assay is calculated from the decrease in absorbance of a pH indicator added to the reaction mixture with a pKi value within the pH range of maximum enzyme activity. Bromocresol green was chosen for our modified method as fungal pectinesterases have an optimum activity at acid pHs (4.6 to 5.1) which are within the pH range of color change (3.8 to 5.4) for this dye. Our methodology was tested by employing a commercial pectinase product as the source of pectinesterases. Statistical analysis using the Student's t-test showed good agreement between the reference titrimetric method and that reported herein.
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