Abstract
A reproducible, critical and simple spectrophotometric method for assay of RNB was investigated is based on (charge transfer complex) formation reaction of RNB with O-Chloranil reagent in the presence of sodium bicarbonate in ethanol to give a highly coloured species with maximal absorption at 440 nm, As well Beer’s law was obeyed in the range of (5-50) µg.mL with a molar absorptivity of 1.21×104 L.mol−1.cm−1 and the limit of detection and quantitation were 0.059 and 0.242 µg.mL respectively. The (average recovery rate) was 101.65% and relative standard deviation (RSD) was better than 0.55%. In addition, it was ratio of 1:1. The method was successfully applied to assay of RNB in Diyala river water and waterproof case.
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